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anti β3 tubulin  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti β3 tubulin
    Anti β3 Tubulin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tubulin/pmc12992974-282-53-55
    Average 86 stars, based on 1 article reviews
    anti β3 tubulin - by Bioz Stars, 2026-09
    86/100 stars

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    other:

    Article Title: Progesterone-driven stabilization of hybrid E/M states in amniotic epithelial cells enhances regeneration and immune modulatory capacities
    Article Snippet: Anti-Tubulin , Cell Signaling , Cat# 3873; RRID: AB_1904178.

    Article Title: Identification of a lineage-agnostic splicing signature caused by PRMT5 inhibition
    Article Snippet: Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Anti-Tubulin, Anti-SDMA, Anti-PRMT5, and Anti-MTAP antibodies were from Cell Signaling Technology (Danvers, MA).

    Article Title: Targeting PI3K γ anchoring enhances CFTR membrane localization and modulator efficacy via PKD1
    Article Snippet: The anti–phospho-Ser/Thr-PKA substrate (catalog 9621S), anti-PKD/PKCμ (catalog 90039), anti-tubulin (catalog 2144), and anti-vinculin (catalog 4650) antibodies were purchased from Cell Signaling Technology.

    Saline:

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM TrisCl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), antiDICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS ARTICLE IN PRESS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti- -actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti- -tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRPconjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRPconjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM Tris- Cl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), anti- DICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr389, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti--actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti--tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRP- conjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRP- conjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Western Blot:

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM TrisCl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), antiDICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS ARTICLE IN PRESS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti- -actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti- -tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRPconjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRPconjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM Tris- Cl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), anti- DICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr389, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti--actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti--tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRP- conjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRP- conjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Article Title: Inducible CD147 up-regulation boosts extended SARS-CoV-2 infection triggering severe COVID-19 independent of ACE2
    Article Snippet: Subsequently, the membranes were blocked with NcmBlot blocking buffer ( P30500 , NCM Biotech) and incubated with the primary antibodies at 4 °C overnight. .. The images were obtained after the incubation of secondary antibodies at room temperature for 1 h. The antibodies used in western blot were listed as follows: anti-Tubulin (3873, CST, dilution 1:3000); anti-GAPDH (60004-1-Ig, Proteintech, dilution 1:50000); anti-Lamin B1 (ab16048, Abcam, dilution 1:5000); anti-ACE2 (AF933, Novus, dilution 1:1000); anti-CD147 (MPZ, Jiangsu Pacific Meinuoke Biopharmaceutical Co., Ltd., dilution 1:2000); anti-CD147 (HAb18, our laboratory, dilution 1:3000); anti-CD147 (orb251620, Biorbyt, dilution 1:2000); anti-AHR (67785-1-Ig, Proteintech, dilution 1:5000); anti-AHRR (CSB- PA028950 , Cusabio, dilution 1:1000); anti-CYP1A1 (13241-1-AP, Proteintech, dilution 1:1000); anti-BCL2 (60178-1-Ig, Proteintech, dilution 1:1000); anti-Caspase3 (66470-1-Ig, Proteintech, dilution 1:3000); anti-Cleaved caspase3 (9661, CST, dilution 1:1000); anti-MLKL (66675-1-Ig, Proteintech, dilution 1:10000); anti-pMLKL (91689, CST, dilution 1:1000); rabbit anti-goat IgG (H + L) (ZB-2306, ZSGB-BIO, dilution 1: 5000); goat anti-human IgG (A0170, Sigma-Aldrich, dilution 1: 5000); goat anti-mouse IgG (H + L) (31430, ThermoFisher Scientific, dilution 1:5000); goat anti-rabbit IgG (H + L) (31460, ThermoFisher Scientific, dilution 1:5000). ..

    Article Title: Granularity screening identifies candidate genes involved in vaccinia virus induced LC3 lipidation
    Article Snippet: Torin1 (MERCK Millipore) was used at 250 nM, 3-Methyladenine (3-MA) (Sigma Aldrich) was used at 5 mM. .. The following antibodies were used: Anti-LC3b (CST #3868S) used in IF at 1:200 and in WB at 1:1000; Anti-Tubulin (CST #9701S) used in WB at 1:5000. .. Hoechst Trihydrochloride Trihydrate 33342 (Invitrogen #H3570) was used for DNA staining in Immunofluorescence (IF) at 1:10,000.

    Incubation:

    Article Title: Inducible CD147 up-regulation boosts extended SARS-CoV-2 infection triggering severe COVID-19 independent of ACE2
    Article Snippet: Subsequently, the membranes were blocked with NcmBlot blocking buffer ( P30500 , NCM Biotech) and incubated with the primary antibodies at 4 °C overnight. .. The images were obtained after the incubation of secondary antibodies at room temperature for 1 h. The antibodies used in western blot were listed as follows: anti-Tubulin (3873, CST, dilution 1:3000); anti-GAPDH (60004-1-Ig, Proteintech, dilution 1:50000); anti-Lamin B1 (ab16048, Abcam, dilution 1:5000); anti-ACE2 (AF933, Novus, dilution 1:1000); anti-CD147 (MPZ, Jiangsu Pacific Meinuoke Biopharmaceutical Co., Ltd., dilution 1:2000); anti-CD147 (HAb18, our laboratory, dilution 1:3000); anti-CD147 (orb251620, Biorbyt, dilution 1:2000); anti-AHR (67785-1-Ig, Proteintech, dilution 1:5000); anti-AHRR (CSB- PA028950 , Cusabio, dilution 1:1000); anti-CYP1A1 (13241-1-AP, Proteintech, dilution 1:1000); anti-BCL2 (60178-1-Ig, Proteintech, dilution 1:1000); anti-Caspase3 (66470-1-Ig, Proteintech, dilution 1:3000); anti-Cleaved caspase3 (9661, CST, dilution 1:1000); anti-MLKL (66675-1-Ig, Proteintech, dilution 1:10000); anti-pMLKL (91689, CST, dilution 1:1000); rabbit anti-goat IgG (H + L) (ZB-2306, ZSGB-BIO, dilution 1: 5000); goat anti-human IgG (A0170, Sigma-Aldrich, dilution 1: 5000); goat anti-mouse IgG (H + L) (31430, ThermoFisher Scientific, dilution 1:5000); goat anti-rabbit IgG (H + L) (31460, ThermoFisher Scientific, dilution 1:5000). ..



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    Servicebio Inc rabbit anti tubulin
    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin <t>O</t> <t>staining</t> of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) <t>and</t> <t>AC-Tub</t> (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.
    Rabbit Anti Tubulin, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti tubulin  (Abmart Inc)
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    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin <t>O</t> <t>staining</t> of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) <t>and</t> <t>AC-Tub</t> (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.
    Anti Tubulin, supplied by Abmart Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tubulin/anti+tubulin+%CE%B1/pm42280634-126-40-41
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    primary antibodies against acetyl α tubulin  (Affinity Biosciences)
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    Affinity Biosciences primary antibodies against acetyl α tubulin
    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin <t>O</t> <t>staining</t> of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) <t>and</t> <t>AC-Tub</t> (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.
    Primary Antibodies Against Acetyl α Tubulin, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+tubulin/anti+tubulin+%CE%B1/pm42262889-783-1-15
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    Image Search Results


    Melatonin dose modulates NSCs lineage commitment, viability, oxidative stress, and mitochondrial membrane potential at day 5. (A) Representative immunofluorescence images of TUJ1 with Nestin in NSCs cultured within the cell-laden MT/BEM matrix and exposed to melatonin (0, 25, 50, 75, 100 μM) for 5 days (scale bar, 50 μm). (B) Representative images of GFAP with Nestin under the same conditions (scale bar, 50 μm). (C) Representative images of Olig2 with Nestin (scale bar, 50 μm). (D) Percentages of TUJ1(+), GFAP (+), and Olig2(+) cells relative to total nuclei (DAPI) (n = 10 fields/group). (E) RT-qPCR of TUJ1, GFAP, and Olig2 normalized to GAPDH and expressed as fold change versus control (ΔΔCt) (n = 6). (F) Live/Dead staining (Calcein AM/EthD-1) at day 5. (G) Western blots of TUJ1 and GFAP with GAPDH loading control. (H) Densitometry of TUJ1/GAPDH and GFAP/GAPDH (n = 3 independent experiments). (I) ROS staining by DCFH-DA with Rosup as the positive control. (J) Quantification of ROS fluorescence intensity (n = 10 fields/group, compared to control). (K) JC-1 staining of mitochondrial membrane potential (ΔΨm) with CCCP as the positive control for depolarization. (L) JC-1 red/green ratio (n = 10 fields/group, compared to control). Statistical analysis: Data are presented as mean ± SD. one-way ANOVA with Holm–Sidak's multiple comparisons for multi-group datasets (D, E, J, L); unpaired two-tailed t -test for the two-group comparison (H). Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Melatonin dose modulates NSCs lineage commitment, viability, oxidative stress, and mitochondrial membrane potential at day 5. (A) Representative immunofluorescence images of TUJ1 with Nestin in NSCs cultured within the cell-laden MT/BEM matrix and exposed to melatonin (0, 25, 50, 75, 100 μM) for 5 days (scale bar, 50 μm). (B) Representative images of GFAP with Nestin under the same conditions (scale bar, 50 μm). (C) Representative images of Olig2 with Nestin (scale bar, 50 μm). (D) Percentages of TUJ1(+), GFAP (+), and Olig2(+) cells relative to total nuclei (DAPI) (n = 10 fields/group). (E) RT-qPCR of TUJ1, GFAP, and Olig2 normalized to GAPDH and expressed as fold change versus control (ΔΔCt) (n = 6). (F) Live/Dead staining (Calcein AM/EthD-1) at day 5. (G) Western blots of TUJ1 and GFAP with GAPDH loading control. (H) Densitometry of TUJ1/GAPDH and GFAP/GAPDH (n = 3 independent experiments). (I) ROS staining by DCFH-DA with Rosup as the positive control. (J) Quantification of ROS fluorescence intensity (n = 10 fields/group, compared to control). (K) JC-1 staining of mitochondrial membrane potential (ΔΨm) with CCCP as the positive control for depolarization. (L) JC-1 red/green ratio (n = 10 fields/group, compared to control). Statistical analysis: Data are presented as mean ± SD. one-way ANOVA with Holm–Sidak's multiple comparisons for multi-group datasets (D, E, J, L); unpaired two-tailed t -test for the two-group comparison (H). Significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Membrane, Immunofluorescence, Cell Culture, Quantitative RT-PCR, Control, Staining, Western Blot, Positive Control, Fluorescence, Two Tailed Test, Comparison

    Three-dimensional immunofluorescence and transcriptomic profiling of melatonin-treated NSCs. (A) Representative 3D confocal reconstructions of NSCs networks cultured for 5 days in BEM or MT/BEM hydrogels, immunostained for Nestin, TUJ1, GFAP and OLIG2 with DAPI nuclear counterstain. Scale bar: 50 μm. (B-C) Quantification of neurite outgrowth showing total neurite length (μm) (B) and neurite filament area (μm 2 ) (C) per field of view. (D) Quantification of astroglial differentiation expressed as GFAP + area (% of ROI). (E) Quantification of oligodendroglial lineage commitment expressed as OLIG2 + cells (% of DAPI + nuclei). Data are presented as mean ± SD. Statistical significance was assessed using an unpaired two-tailed t -test; ∗p < 0.05, ∗∗p < 0.01 versus NSCs@BEM.

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Three-dimensional immunofluorescence and transcriptomic profiling of melatonin-treated NSCs. (A) Representative 3D confocal reconstructions of NSCs networks cultured for 5 days in BEM or MT/BEM hydrogels, immunostained for Nestin, TUJ1, GFAP and OLIG2 with DAPI nuclear counterstain. Scale bar: 50 μm. (B-C) Quantification of neurite outgrowth showing total neurite length (μm) (B) and neurite filament area (μm 2 ) (C) per field of view. (D) Quantification of astroglial differentiation expressed as GFAP + area (% of ROI). (E) Quantification of oligodendroglial lineage commitment expressed as OLIG2 + cells (% of DAPI + nuclei). Data are presented as mean ± SD. Statistical significance was assessed using an unpaired two-tailed t -test; ∗p < 0.05, ∗∗p < 0.01 versus NSCs@BEM.

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Immunofluorescence, Cell Culture, Two Tailed Test

    Molecular validation of neural repair and mechanism activation in spinal cord tissue. Western blot and qPCR analyses of spinal cord tissue lysates from Sham, SCI, BEM, NSCs@BEM, and NSCs@MT/BEM groups. (A) Representative Western blots for the neuronal marker TUJ1 and the glial scar marker GFAP. (B) Representative Western blots for phosphorylated AMPK (p-AMPK), phosphorylated ACC (p-ACC), and their respective total proteins. (C) Representative Western blots for the five oxidative phosphorylation (OXPHOS) complex subunits. (D) Densitometric quantification of TUJ1 and GFAP protein levels. (E) Densitometric quantification of the p-AMPK/total AMPK and p-ACC/total ACC ratios. (F) Densitometric quantification of OXPHOS complex protein levels. (G) Relative mRNA expression of neural markers (TUJ1, GFAP, Olig2) and key mitochondrial biogenesis regulators (Ppargc1a, Tfam) determined by qPCR. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA with Holm–Sidak's multiple comparisons test. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

    Journal: Bioactive Materials

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury

    doi: 10.1016/j.bioactmat.2026.04.006

    Figure Lengend Snippet: Molecular validation of neural repair and mechanism activation in spinal cord tissue. Western blot and qPCR analyses of spinal cord tissue lysates from Sham, SCI, BEM, NSCs@BEM, and NSCs@MT/BEM groups. (A) Representative Western blots for the neuronal marker TUJ1 and the glial scar marker GFAP. (B) Representative Western blots for phosphorylated AMPK (p-AMPK), phosphorylated ACC (p-ACC), and their respective total proteins. (C) Representative Western blots for the five oxidative phosphorylation (OXPHOS) complex subunits. (D) Densitometric quantification of TUJ1 and GFAP protein levels. (E) Densitometric quantification of the p-AMPK/total AMPK and p-ACC/total ACC ratios. (F) Densitometric quantification of OXPHOS complex protein levels. (G) Relative mRNA expression of neural markers (TUJ1, GFAP, Olig2) and key mitochondrial biogenesis regulators (Ppargc1a, Tfam) determined by qPCR. Data are presented as mean ± SD. Statistical significance was determined by one-way ANOVA with Holm–Sidak's multiple comparisons test. (∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001).

    Article Snippet: Following blocking, membranes were incubated overnight at 4 °C with the following primary antibodies: GFAP (1:1000; #80788, CST, USA), TUJ1 (1:1000; #YA586, MCE, China), GAPDH (1:1000; #2118, CST, USA), phospho-ACC (Ser79) (1:1000; #11818, CST, USA), Acetyl-CoA Carboxylase (C83B10) (1:1000; #3676, CST, USA), Total OXPHOS Rodent WB Antibody Cocktail (1:1000; #ab110413, Abcam, UK), phospho-AMPKα (Thr172) (1:1000; #2535, CST, USA), and total AMPKα (1:1000; #2532, CST, USA).

    Techniques: Biomarker Discovery, Activation Assay, Western Blot, Marker, Phospho-proteomics, Expressing

    Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

    Journal: Bioactive Materials

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

    doi: 10.1016/j.bioactmat.2026.01.026

    Figure Lengend Snippet: Gel-AgNA/MgGA MN promote mucosal regeneration. (a) HE and Safranin O staining of rabbit tracheal samples harvested at Day 10 post-operation after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b) IF staining of CK14 (marker of basal cells, red) and AC-Tub (marker of cilia cell, green). (c) IF staining of ZO-1 (marker of tight junctions, orange). (d, e) Quantitative analysis of regenerated epithelial coverage and thickness (n = 9). (f) Masson and Sirius Red staining for collagen evaluation after various treatments (n = 5). Quantitative analysis of collagen volume fraction (g) and fiber orientation (h) . The pentagram indicates luminal side of trachea.

    Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

    Techniques: Staining, Marker